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Description

Briefly, primary cells were washed with PBS, lysed in 100 l of 0.4 N perchloric acid (PCA) for 30 min at 4 C, and centrifuged, and the supernatants were neutralized with four volumes of 0.1 M NaH 2 PO 4 and 5 mM EDTA, pH 7.5

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